In-House Chemistries

Titansphere®

TiO2 / 5 µm / 100 Å
Titansphere is a relatively new base material for HPLC analysis developed byGL Sciences consisting of spherical particles of titanium dioxide (TiO2). Titansphere has been found to be particularly effective in the selective enrichment of phosphorylated peptides from proteolytic digests, offering greater selectivity for phosphopeptides than alternative methods, such as IMAC (immobilized metal affinity chromatography). Typically, IMAC shows significant binding of non-phosphorylated peptides which have multiple acidic residues. This problem is easily eliminated by using Titansphere.

Typically, a Titansphere column is used to selectively bind phosphorylated peptides from a peptide mixture. The phosphorylated peptides are then eluted from the column onto a reverse-phase column for further characterization or analyzed directly by MS.


GL Sciences

Specifications:


Media Code: H210


<< Return to Chemistry Index


Don't see the particular chemistry you need?

Custom packing is available with just about any chemistry you require. Please contact us for more details; via email, phone +1 781.933.9560, or fax +1.781.933.9564.